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非放射性蛋白激酶测试盒

发布:admin 发布时间:2018-06-01 来源:

生物体内普遍存在的信息转导调节方式是蛋白激酶(protein kinase, pk)和蛋白磷酸酶(protein phosphatase) 催化蛋白质磷酸化与去磷酸化,这一过程几乎涉及所有的生理及病理过程,如糖代谢、光合作用、细胞的生长发育、基因表达、神经递质的合成与释放,甚至癌变等等,在细胞信号转导过程中占有极其重要的位置。

我们提供ImmuneChem公司的非放射性同位素蛋白激酶活性分析测试盒:该产品是许多世界级制药公司一直投入大量的人力物力进行开发的新产品。ICP所开发成功的蛋白激酶活性测试盒属世界上首创,安全、快速、灵敏度高,稳定。

并成功开发了广谱固相非放射性同位素生色反应蛋白激酶活性测试盒,可用于数十种重要的纯化蛋白激酶的活性测试及其抑制剂的测试(可用于高通量药物筛选)

 

产品名称货号规格应用价格产地/品牌

PKA Kinase Assay Kits, Type 

(蛋白激酶A活性分析测试盒I型)

ICP0216
96T
PKA Kinase Activity Assay¥4350元加拿大 Immunechem

PKA Kinase Assay Kits, Type I

(蛋白激酶A活性分析测试盒II型)

ICP021796TPKA Kinase Activity Assay¥4350元加拿大 Immunechem

PKB Kinase Assay Kits, Type I

(蛋白激酶B活性分析测试盒I型)

ICP024596TPKB Kinase Activity Assay¥4350元加拿大 Immunechem

PKB(AKT) Kinase Assay Kits, Type II

(蛋白激酶B活性分析测试盒II型)

ICP0243
96TPKB(AKT) Kinase Assay¥4350元加拿大 Immunechem

PKC Kinase Assay Kits, Type I

(蛋白激酶C活性分析测试盒I型)

ICP021296T PKC Activity Assay¥4350元加拿大 Immunechem

PKC Kinase Assay Kits, Type II

(蛋白激酶C活性分析测试盒II型)

ICP021196T PKC Activity Assay¥4350元加拿大 Immunechem

S6K Kinase Assay Kits, Type I

(核糖体蛋白S6激酶活性分析测试盒I型)

ICP025596TS6K Activity Assay¥4350元加拿大 Immunechem

SGK Kinase Assay Kits, Type II

(血清和糖皮质激素调节蛋白激酶活性分析测试盒II型)

ICP0242
96TSGK Kinase Activity Assay¥4350元加拿大 Immunechem


  蛋白质丝氨酸/苏氨酸激酶检测试剂盒

(Protein Serine/Threonine Kinase, STK)


ICP0248

96T

广谱检测
¥4350元

加拿大 Immunechem

蛋白质酪氨酸激酶检测试剂盒

(Protein Tyrosine Kinase, PTK)

96T广谱检测即将上市加拿大 Immunechem
Catalog #:ICP0248Broad Specific Kinase Assay Kit    
Product DescriptionThis product is designed to detect the activity for variety of kinases (see Table 1) and screening the inhibitors of the corresponding kinase. The assay kit is non-radioactive but colorimetric.
The principle of the assay is that:
The kinase substrates is immobilized onto the 96-well plate. The kinases use ATP to phosphorylate the substrate on the plate. The phosphorylated product is detected by the phosphor-substrate specific antibodies. See figure1 for the principle illustration.
Formulation1x96 assays or 5x96 assays
ApplicationsAssay for the pure kinases listed in Table 1
Assay for inhibitors of the serine/threonine kinases listed in Table 1
Storage & StabilityProduct is stable for 30 days at room remperature. 6 months at 4°C.
Kit Compoentes1.96-well Substrate Microtiter Plate (Part#: KA101)
2.Phosphospecific Substrate Antibody(Part#: KA102)
3.Anti-Rabbit IgG: HRP Conjugate(Part#: KA103)
4.Antibody Dilution Buffer (Part#:KA104)
5.Kinase Assay Dilution Buffer (Part#:KA105)
6.ATP(Part#: KA106)
7.20xWash Buffer (Part#:KA107)
8.TMB Substrate (Part#:KA108)
9.Stop Solution  (Part#:KA109)

 

Table 1: Kanise* activity tested by the Assay Kit
No.KinaseActivityNo.KinaseActivityNo.KinaseActivity
1AKT1+++++32CLK2+++++63PKAc beta+++++
2AKT2+++++33CLK3+++64PKAc gamma+++++
3AKT3+++++34CLK4+++65PKAc mu+++++
4AMPK (A1/B1/G1)+++++35DAPK1++++66PKAc nu+++++
5AMPK (A1/B1/G2)+++++36DAPK2++++67PKAc zeta+++++
6AMPK (A1/B1/G3)++++37DAPK3++68PKD2++++
7AMPK (A1/B2/G1)+++++38DCAMKL2++69PKN2/PRK2++++
8AMPK (A1/B2/G2)+++++39ERK5++++70PLK3+++
9AMPK (A1/B2/G3)+++++40KSR2+++++71PLK4 (SAK)+++
10AMPK (A2/B1/G1)+++++41MAK++++72PRKG1+++++
11AMPK (A2/B1/G2)+++++42MAPKAPK2+++++73PRKG2++++
12AMPK (A2/B1/G3)+++++43MAPKAPK3++++74PRKX+++++
13AMPK (A2/B2/G1)+++++44MAPKAPK5+++75RIPK3++++
14AMPK (A2/B2/G2)++++45MARK1+++76RSK1+++++
15AMPK (A2/B2/G3)++++46MARK2++++77RSK2++++
16AURORA A+++++47MARK3++++78RSK3++++
17AURORA B++++48MARK4++79RSK4+++
18AURORA C++++49MELK++++80SBK1+++++
19BMPR2+++50MLK1+++81SGK+++
20CAMK1 beta++++51MNK1++++82SGK2+++++
21CAMK1 delta++52MNK2++++83SIK++++
22CAMK1 gamma++53MSK1+++++84SIK3++++
23CAMK2 alpha++54MYLK2+++85STK39 (STLK3)++++
24CAMK2 beta++++55Nuak2 (SNARK)++++86TBK1+++
25CAMK2 delta++56p70S6K+++87TSSK1B++++
26CAMK4++++57p70S6Kb+++88TSSK2+++++
27CDK6/CyclinD3++++58PAK3++++89ULK1+++
28CDK7/CyclinH1/MNAT1++++59PIM1++90ULK2+++
29CHK1+++++60PIM2++++91ULK3++++
30CHK2++++61PIM3++92WNK1++
31CLK1+++++62PKAc alpha++++

 

 

 

测试盒原理:

 基于ELISA的基本原理。PKC底物被包被在酶标板上,在ATP存在的前提下,PKC激酶将底物磷酸化,洗掉游离的PKC激酶和其他成分。再加入特异性磷酸化抗体(只识别磷酸化底物,而与非磷酸化底物无交叉反应。)检测残留在酶标板上的磷酸化底物。通过酶标二抗放大信号,读出OD值(蛋白激酶活性与OD值的对数成正比)。与对照组相比,得出处理组的蛋白激酶活性。

文献引用:

PKC试剂盒的引用:Biochemical and Biophysical Research Communications

Volume 425, Issue 2, 24 August 2012, Pages 182–188

PKB 试剂盒的引用:April 22, 2011 The Journal of Biological Chemistry, 286, 13995-14006.

PKA 试剂盒的引用:Cancer Res September 15, 2003 63; 596