发布：admin 发布时间：2018-10-29 来源：本站

蛋白激酶和磷酸酶对很多生物机制起着关键的调节作用，一般通过蛋白的磷酸化和去磷酸化调节诸如细胞生长、增殖、分化、代谢等生理过程，而细胞信号转导中激酶和磷酸酶的活化异常将导致多种人类疾病。

目前，已经发现人体内含有超过600种激酶和磷酸酶，而快速筛选并开发针对这些酶类的潜在治疗药物，具有非常巨大的经济价值，FDA已经批准超过30种激酶类抑制剂药物。

加拿大munechem公司新上市的广谱‍固相非放射性同位素生色反应蛋白激酶活性测试盒，可用于数十种重要的纯化蛋白激酶的活性测试及其抑制剂的测试（用于高通量药物筛选）

Broad Specific Kinase Assay Kit

Catalog # ICP0248

Pack Size：96 assays

Product Description			This product is designed to detect the activity for variety of kinases (see Table 1) and screening the inhibitors of the corresponding kinase. The assay kit is non-radioactive but colorimetric. The principle of the assay is that: The kinase substrates is immobilized onto the 96-well plate. The kinases use ATP to phosphorylate the substrate on the plate. The phosphorylated product is detected by the phosphor-substrate specific antibodies. See figure1 for the principle illustration.
Formulation			1x96 assays or 5x96 assays
Applications			Assay for the pure kinases listed in Table 1 Assay for inhibitors of the serine/threonine kinases listed in Table 1
Storage & Stability			Product is stable for 30 days at room remperature. 6 months at 4°C.
Kit Compoentes			1. 96-well Substrate Microtiter Plate (Part#: KA101) 2. Phosphospecific Substrate Antibody(Part#: KA102) 3. Anti-Rabbit IgG: HRP Conjugate(Part#: KA103) 4. Antibody Dilution Buffer (Part#:KA104) 5. Kinase Assay Dilution Buffer (Part#:KA105) 6. ATP(Part#: KA106) 7. 20X Wash Buffer (Part#:KA107) 8. TMB Substrate (Part#:KA108) 9. Stop Solution (Part#:KA109)


*Table 1: Kanise activity tested by the Assay Kit**
No.	Kinase	Activity	No.	Kinase	Activity	No.	Kinase	Activity
1	AKT1	+++++	32	CLK2	+++++	63	PKAc beta	+++++
2	AKT2	+++++	33	CLK3	+++	64	PKAc gamma	+++++
3	AKT3	+++++	34	CLK4	+++	65	PKAc mu	+++++
4	AMPK (A1/B1/G1)	+++++	35	DAPK1	++++	66	PKAc nu	+++++
5	AMPK (A1/B1/G2)	+++++	36	DAPK2	++++	67	PKAc zeta	+++++
6	AMPK (A1/B1/G3)	++++	37	DAPK3	++	68	PKD2	++++
7	AMPK (A1/B2/G1)	+++++	38	DCAMKL2	++	69	PKN2/PRK2	++++
8	AMPK (A1/B2/G2)	+++++	39	ERK5	++++	70	PLK3	+++
9	AMPK (A1/B2/G3)	+++++	40	KSR2	+++++	71	PLK4 (SAK)	+++
10	AMPK (A2/B1/G1)	+++++	41	MAK	++++	72	PRKG1	+++++
11	AMPK (A2/B1/G2)	+++++	42	MAPKAPK2	+++++	73	PRKG2	++++
12	AMPK (A2/B1/G3)	+++++	43	MAPKAPK3	++++	74	PRKX	+++++
13	AMPK (A2/B2/G1)	+++++	44	MAPKAPK5	+++	75	RIPK3	++++
14	AMPK (A2/B2/G2)	++++	45	MARK1	+++	76	RSK1	+++++
15	AMPK (A2/B2/G3)	++++	46	MARK2	++++	77	RSK2	++++
16	AURORA A	+++++	47	MARK3	++++	78	RSK3	++++
17	AURORA B	++++	48	MARK4	++	79	RSK4	+++
18	AURORA C	++++	49	MELK	++++	80	SBK1	+++++
19	BMPR2	+++	50	MLK1	+++	81	SGK	+++
20	CAMK1 beta	++++	51	MNK1	++++	82	SGK2	+++++
21	CAMK1 delta	++	52	MNK2	++++	83	SIK	++++
22	CAMK1 gamma	++	53	MSK1	+++++	84	SIK3	++++
23	CAMK2 alpha	++	54	MYLK2	+++	85	STK39 (STLK3)	++++
24	CAMK2 beta	++++	55	Nuak2 (SNARK)	++++	86	TBK1	+++
25	CAMK2 delta	++	56	p70S6K	+++	87	TSSK1B	++++
26	CAMK4	++++	57	p70S6Kb	+++	88	TSSK2	+++++
27	CDK6/CyclinD3	++++	58	PAK3	++++	89	ULK1	+++
28	CDK7/CyclinH1/MNAT1	++++	59	PIM1	++	90	ULK2	+++
29	CHK1	+++++	60	PIM2	++++	91	ULK3	++++
30	CHK2	++++	61	PIM3	++	92	WNK1	++
31	CLK1	+++++	62	PKAc alpha	++++